DNA Flow-Stretch Assays for Studies of Protein-DNA Interactions at the Single-Molecule Level

Protein-DNA interactions are the core of cell’s molecular machinery. For a long time, conventional biochemical methods served as powerful investigatory basis protein-DNA and target search mechanisms. Currently single-molecule (SM) techniques have emerged complementary tool for studying these revealed plenty previously obscured mechanistic details. In comparison to traditional ones, SM allow direct monitoring individual biomolecules. Therefore, reveal reactions that otherwise hidden by ensemble averaging observed in bulk-type methods. biophysical employing various nanobiotechnology immobilization studied molecules grant possibility monitor reaction trajectories Next-generation vitro biophysics approaches enabling high-throughput studies characterized much greater complexity than ones developed previously. Currently, several DNA flow-stretch assays been published shown many benefits DNA-binding proteins, such CRISPR-Cas, Argonaute, ATP-fueled helicases translocases, others. This review focuses on surface-immobilized relatively interaction